At Robust Diagnostics, we’re experts at measuring epitope level antibody response using peptide microarrays.
Our focus is on developing information rich peptide microarray assays that help our customers solve their challenging immune response monitoring projects. With a focus on sensitivity, quality, and reproducibility, we produce microarrays that monitor antibody binding to up to 150 epitopes in a single assay.
Antibody binding to peptide epitopes is commonly measured using direct ELISA assays in which the peptide epitope of interest is coated in the well of a 96-well plate before the antibody containing sample was added. Antibody binding is then measured using a secondary antibody labeled with a reporter dye or enzyme. This method is incredibly efficient and sensitive with protein antigens but due to the different chemical properties of peptides, peptide antigens can have low sensitivity and low reproducibility.
Robust Diagnostics overcomes these challenges by preparing peptide microarrays that use covalent immobilization of peptides to planar surfaces combined with high sensitivity fluorescence antibody detection technology to produce highly sensitive and reproducible assays. Peptide microarrays have the added benefit of multiplexing and up to 150 peptide antigens can be measured in a single assay. The method requires small sample volumes (<10 uL) and produces information rich datasets ideal for biomarker discovery, validation, or as input for computational approaches.
We work closely with our customers to understand assay requirements so that we can design the assay and workflow that meets their specific needs.
Once design requirements are finalized, Robust Diagnostics produces the customer microarrays and processes customer samples in our laboratory according to the agreed upon experimental design. Experimental data is provided to the customer through a secure data room.